Yoshika makes a sortie on her own, in an effort to protect Britannia. However, she is severely outnumbered and outgunned by the enemy, when her teammates from the 501st Joint Fighter Wing: Strike Witches come to the rescue!

The First Special Assault Force, a.k.a. the Warlock, is assigned to Britannia as a new anti-Neuroi force. Minna and some others, who had been suspicious of the maneuverings of the top officials, begin to investigate on their own.

In her encounter with Neuroi, Yoshika makes an unauthorized decision. As a result, Minna grounds her and confines her to her room for a certain period of time. However, a pressing matter arises which leaves Yoshika no choice but to sneak out of her room. As she leaves her room, she runs into her friend Lynne who came to see if Yoshika was okay....

Yoshika pulls off an impressive combat tactic and wins a simulated dogfight. The rapid improvement in Yoshika’s skills annoys Perrine, who suspects that Sakamoto must have secretly given Yoshika personal training sessions allowing her to gain special skills. Driven by competitive envy, Perrine challenges Yoshika.

Akagi, the aircraft carrier that transported Yoshika from the Empire of Fuso, makes a port call at Port Witches. The crew hands Yoshika a thank-you letter which is snatched away by Minna. Puzzled by Minna’s strange behavior, Yoshika seeks her best friend Lynne’s advice.

One morning, Erica Hartmann finds part of her uniform missing. Nonchalantly strolling into the locker room, she puts on someone else’s uniform and goes on duty which causes much commotion within the team. To further complicate things, the team is summoned to a scramble!

Yoshika and her comrades are attacked by the Neurois on their way back from a mission in London, but gets help from Sanya who happened to be on a night patrol. As a result, Yoshika is given orders to serve the night shift, and must spend the night in the same room with Sanya, when an envious Eila barges in.

Shirley the speed freak is obsessed with trying to exceed sonic speed. Busybody Francesca Luccini meddles with the adjustments of Shirley’s Striker Unit before the water training drill required by all members, which subsequently leads to an unexpected incident.

Yoshika gradually orients herself to life as a member of Strike Witches. However, she is condemned by her supervisor Gertrud Barkhorn as being inexperienced. Desperate to win her acceptance, Yoshika gets carried away in her training when she suddenly comes face to face with the Neuroi.

Yoshika makes her decision to join the Strike Witches. Lynette Bishop, another rookie witch who is 15 years old, shows Yoshika around. Although Yoshika is nervous about her new assignment and understands nothing about the arrangement of the troop, Lynette acts in a somewhat unfriendly manner, adding to Yoshika’s anxiety.

On their journey to Britannia, Yoshika and the others are assaulted by mysterious invaders called the Neuroi, menacing beings from another world. In order to buy time until reinforcements arrive, Major Sakamoto sallies forth alone, only to be met by a fierce attack from the opposition. Despite her best efforts she is driven back and ultimately cornered. Yoshika can do nothing but wait in terror inside the battleship.

Yoshika Miyafuji is a fourteen-year-old girl who possesses magical powers that can heal. One day she is approached by Mio Sakamoto, Major of the 501st Joint Fighter Wing a.k.a. Strike Witches to join her team. A few days after Yoshika turns down the invitation because of her abhorrence toward wars, she receives a letter.